Journal: International Journal of Biological Sciences
Article Title: Pyrroloquinoline Quinone Prevents Estrogen Deficiency-Induced Osteoporosis by Inhibiting Oxidative Stress and Osteocyte Senescence
doi: 10.7150/ijbs.25783
Figure Lengend Snippet: Effects of PQQ and estrogen on osteocyte senescence and SASP in OVX mice. Representative micrographs from paraffin sections of distal femurs with immunohistochemical staining for (A) p16 and (B) β-gal. Scale bars represent 50 μm in A and B. (C) The percentage of p16 positive osteocyte cells and (D) β-gal positive cells. (E) mRNA expression of established SASP components. Serum pro-inflammatory cytokines (F) TNF-α, (G) IL-1β, and (H) IL-6 were measured. Data were the means ± SEM (n = 6 for each group). #P < 0.05 and ##P < 0.01, vs. the sham group. *P < 0.05 and **P < 0.01, vs. the OVX-Veh group.
Article Snippet: Immunohistochemical staining was carried out as described previously for β-galactosidase (β-gal), P16 INK4a , using the avidin-biotin-peroxidase complex technique with affinity-purified rabbit anti-mouse β-gal (Santa Cruz, CA, USA), P16 INK4a (Santa 192Cruz, CA, USA).
Techniques: Immunohistochemical staining, Staining, Expressing